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Probing tissue interaction with laser-based cauterization in the early developing Drosophila embryo

Abstract : Tissue morphogenesis is governed by mechanical forces generated by cell cytoskeletal networks. It has been shown that subcellular forces are responsible for cell shape changes. Nevertheless cells in a developing organism do not act in isolation: cells contact and adhere one another, and forces are transmitted from cell-to-cell throughout tissues. Understanding how forces are integrated at the tissue level and finally at the full animal scale is nowadays a major challenge that will allow shedding new light on how embryo morphogenesis takes place. In this chapter, I present a new laser-based technique to probe tissue coupling in a living Drosophila embryo. Such technique allows generating mechanical fix boundaries that can eventually impair or modulate cell flows and tissue displacements to probe tissue interaction.
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https://hal.univ-cotedazur.fr/hal-02871166
Contributor : Caroline Ruiz <>
Submitted on : Wednesday, June 17, 2020 - 10:01:57 AM
Last modification on : Thursday, June 18, 2020 - 3:27:13 AM

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M. Rauzi. Probing tissue interaction with laser-based cauterization in the early developing Drosophila embryo. Methods in Cell Biology, 139, pp.153-165, 2017, ⟨10.1016/bs.mcb.2016.11.003⟩. ⟨hal-02871166⟩

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