The emerging landscape of single-molecule protein sequencing technologies
Javier Antonio Alfaro
(1)
,
Peggy Bohländer
(2)
,
Mingjie Dai
(3, 4)
,
Mike Filius
(2)
,
Cecil J. Howard
(5)
,
Xander F. Van Kooten
(6)
,
Shilo Ohayon
(6)
,
Adam Pomorski
(2)
,
Sonja Schmid
(7)
,
Aleksei Aksimentiev
(8)
,
Eric V. Anslyn
(5)
,
Georges Bedran
(1)
,
Chan Cao
(9)
,
Mauro Chinappi
(10)
,
Etienne Coyaud
(11, 12)
,
Cees Dekker
(2)
,
Gunnar Dittmar
(13, 14)
,
Nicholas Drachman
(15)
,
Rienk Eelkema
(2)
,
David Goodlett
(1, 16)
,
Sébastien Hentz
(17, 18)
,
Umesh Kalathiya
(1)
,
Neil L. Kelleher
(19)
,
Ryan T. Kelly
(20)
,
Zvi Kelman
(21)
,
Sung Hyun Kim
(2)
,
Bernhard Kuster
(22)
,
David Rodriguez-Larrea
(23)
,
Stuart Lindsay
(24)
,
Giovanni Maglia
(25)
,
Edward M. Marcotte
(5)
,
John P. Marino
(21)
,
Christophe D. Masselon
(26)
,
Michael Mayer
(27)
,
Patroklos Samaras
(22)
,
Kumar Sarthak
(8)
,
Lusia Sepiashvili
(28)
,
Derek Stein
(15)
,
Meni Wanunu
(29)
,
Mathias Wilhelm
(22)
,
Peng Yin
(3, 4)
,
Amit Meller
(6)
,
Chirlmin Joo
(2)
1
UG -
University of Gdańsk
2 TU Delft - Delft University of Technology
3 Harvard University
4 HMS - Harvard Medical School [Boston]
5 University of Texas at Austin [Austin]
6 Technion - Israel Institute of Technology [Haifa]
7 WUR - Wageningen University and Research [Wageningen]
8 UIUC - University of Illinois at Urbana-Champaign [Urbana]
9 EPFL - Ecole Polytechnique Fédérale de Lausanne
10 Università degli Studi di Roma Tor Vergata [Roma] = University of Rome Tor Vergata
11 CHRU Lille - Centre Hospitalier Régional Universitaire [CHU Lille]
12 PRISM - Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192
13 LIH - Luxembourg Institute of Health
14 University of Luxembourg [Luxembourg]
15 Brown University
16 UVIC - University of Victoria [Canada]
17 CEA-LETI - Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information
18 UGA - Université Grenoble Alpes
19 Northwestern University [Evanston]
20 BYU - Brigham Young University
21 IBBR - Institute for Bioscience and Biotechnology Research [Rockville, MD, États-Unis]
22 TUM - Technische Universität Munchen - Technical University Munich - Université Technique de Munich
23 UPV/EHU - Universidad del Pais Vasco / Euskal Herriko Unibertsitatea [Espagne]
24 ASU - Arizona State University [Tempe]
25 University of Groningen [Groningen]
26 BGE - UMR S1038 - Laboratoire de Biologie à Grande Échelle
27 UNIFR - Université de Fribourg = University of Fribourg
28 University of Toronto
29 Northeastern University [Boston]
2 TU Delft - Delft University of Technology
3 Harvard University
4 HMS - Harvard Medical School [Boston]
5 University of Texas at Austin [Austin]
6 Technion - Israel Institute of Technology [Haifa]
7 WUR - Wageningen University and Research [Wageningen]
8 UIUC - University of Illinois at Urbana-Champaign [Urbana]
9 EPFL - Ecole Polytechnique Fédérale de Lausanne
10 Università degli Studi di Roma Tor Vergata [Roma] = University of Rome Tor Vergata
11 CHRU Lille - Centre Hospitalier Régional Universitaire [CHU Lille]
12 PRISM - Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192
13 LIH - Luxembourg Institute of Health
14 University of Luxembourg [Luxembourg]
15 Brown University
16 UVIC - University of Victoria [Canada]
17 CEA-LETI - Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information
18 UGA - Université Grenoble Alpes
19 Northwestern University [Evanston]
20 BYU - Brigham Young University
21 IBBR - Institute for Bioscience and Biotechnology Research [Rockville, MD, États-Unis]
22 TUM - Technische Universität Munchen - Technical University Munich - Université Technique de Munich
23 UPV/EHU - Universidad del Pais Vasco / Euskal Herriko Unibertsitatea [Espagne]
24 ASU - Arizona State University [Tempe]
25 University of Groningen [Groningen]
26 BGE - UMR S1038 - Laboratoire de Biologie à Grande Échelle
27 UNIFR - Université de Fribourg = University of Fribourg
28 University of Toronto
29 Northeastern University [Boston]
Etienne Coyaud
- Fonction : Auteur
- PersonId : 1180207
- IdHAL : etienne-coyaud
- ORCID : 0000-0002-5893-4557
Sébastien Hentz
- Fonction : Auteur
- PersonId : 785834
- ORCID : 0000-0003-0479-9027
- IdRef : 076620484
Christophe D. Masselon
- Fonction : Auteur
- PersonId : 173519
- IdHAL : christophe-masselon
- ORCID : 0000-0003-0117-2480
- IdRef : 180969331
Résumé
Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.
Domaines
Sciences du Vivant [q-bio]
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