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Theses

Détermination de résidus de médicaments et ses métabolites dans les plantes sauvages. Essais d’exposition et évaluation de la bioactivité

Abstract : The presence of pharmaceuticals in environmental waters or plant matrices suppose a challenge for environment preservation, which makes necessary to evaluate, monitor and carry out an environmental diagnosis of the areas affected by pollution in order to know the ecosystems damaged by the presence of pollutants and thus evaluate the consequences of these on living beings. For this reason, this Thesis focuses its study on the effects of pollution on the native flora of the Doñana National Park (Spain), providing qualitative and quantitative data on the effect of pharmacologically active substances (such as emerging contaminants) in three plant species in this area, in addition similar studies are virtually nonexistent. This work aims to increase knowledge about the consequences of anthropogenic pressure in the ecosystem of Doñana National Park, simulating at laboratory scale the presence of pharmaceuticals, their degradation compounds and metabolites, in plant matrices from this natural environment. The antioxidant and enzymatic capacity as well as the identification of inhibitory molecules to the enzymes used in this study were evaluated in a collection of 150 selected plants from Doñana. An analytical procedure using high resolution liquid chromatography with detection of mass spectrometry - flight time has been optimized and validated for the identification and quantification of six active pharmacological principles, carbamazepine, flumequine, ciprofloxacin, enrofloxacin, ibuprofen and diclofenac, in extracts from three plant species: Lavandula dentata, Juncus sp. and Salicornia europaea. An uptake study has been carried out, irrigating the selected plants with aqueous solutions containing variable concentrations of the pharmaceuticals. The samples have been analyzed at 7, 15, 21 and 30 days (end of the assay) and the accumulation of the selected substances have been determined. Additionally, non-targeted analytical techniques using appropriate software (MetabolynxTM) have been applied to data from the samples subjected to the uptake study to identify possible metabolites and degradation products and possible metabolic routes involved. Additionally, some methods were optimized to evaluate antioxidant and inhibitory activities, using different enzymes (tyrosinase, elastase, collagenase and lipoxygenase), in the extracts of a selection of 150 plants from the Doñana National Park. For this purpose, the specimens were extracting using solvents of different polarity and extracting capacity: hexane, ethanol, water: ethanol (1:1, v/v) and ethyl acetate. These methods were applied to evaluate the enzymatic inhibition capacity as well as antioxidant capacity of the extracts.
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  • HAL Id : tel-03232977, version 1

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Sofía Barreales Suárez. Détermination de résidus de médicaments et ses métabolites dans les plantes sauvages. Essais d’exposition et évaluation de la bioactivité. Chimie analytique. COMUE Université Côte d'Azur (2015 - 2019); Universidad de Sevilla (Espagne), 2019. Français. ⟨NNT : 2019AZUR4086⟩. ⟨tel-03232977⟩

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