Outils d'analyse d'images et recalage d'individus pour l'étude de la morphogenèse animale et végétale

Gaël Michelin 1
1 MORPHEME - Morphologie et Images
CRISAM - Inria Sophia Antipolis - Méditerranée , IBV - Institut de Biologie Valrose : U1091, Laboratoire I3S - SIS - Signal, Images et Systèmes
Abstract : In developmental biology, the study of model organisms aims for theunderstanding of genetic mechanisms responsible of morphogenesis. Today,fluorescent confocal microscopy is a means for in vivo imaging of developingorganisms at cell level with a high spatio-temporal resolution. To handle such3D+t image sequences, adapted computer-assisted methods are highlydesirable.In this thesis, we build dedicated tools for the study of two developingorganisms, the ascidian Phallusia mammillata's embryo and the Arabidopsisthaliana's floral meristem.We first develop a method for segmentation comparison adapted to developingorganism epithelial tissue images. This tool is then used to validate our secondcontribution that is about the development of a cell membranes detection andreconstruction tool for cell shape segmentation process applied to ascidian andarabidopsis images.We then use the previously introduced membrane detection tool to build aninter-individual spatial registration strategy applied to ascidian embryo images.Finally, we develop an inter-individual spatio-temporal registration strategyapplied to 3D image sequences of arabidopsis floral meristems
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Gaël Michelin. Outils d'analyse d'images et recalage d'individus pour l'étude de la morphogenèse animale et végétale. Autre. Université Côte d'Azur, 2016. Français. ⟨NNT : 2016AZUR4088⟩. ⟨tel-01451608⟩

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