Skip to Main content Skip to Navigation
Journal articles

The Drosophila actin nucleator DAAM is essential for left-right asymmetry

Abstract : Left-Right (LR) asymmetry is essential for organ positioning, shape and function. Myosin 1D (Myo1D) has emerged as an evolutionary conserved chirality determinant in both Drosophila and vertebrates. However, the molecular interplay between Myo1D and the actin cytoskeleton underlying symmetry breaking remains poorly understood. To address this question, we performed a dual genetic screen to identify new cytoskeletal factors involved in LR asymmetry. We identified the conserved actin nucleator DAAM as an essential factor required for both dextral and sinistral development. In the absence of DAAM, organs lose their LR asymmetry, while its overexpression enhances Myo1D-induced de novo LR asymmetry. These results show that DAAM is a limiting, LR-specific actin nucleator connecting up Myo1D with a dedicated F-actin network important for symmetry breaking.
Document type :
Journal articles
Complete list of metadatas

https://hal.univ-cotedazur.fr/hal-02872101
Contributor : Caroline Ruiz <>
Submitted on : Monday, February 8, 2021 - 10:14:53 AM
Last modification on : Friday, February 12, 2021 - 8:07:30 AM

File

pgen.1008758.pdf
Publication funded by an institution

Identifiers

Citation

Anil Chougule, François Lapraz, István Földi, Delphine Cérézo, József Mihály, et al.. The Drosophila actin nucleator DAAM is essential for left-right asymmetry. PLoS Genetics, Public Library of Science, 2020, 16 (4), pp.e1008758. ⟨10.1371/journal.pgen.1008758⟩. ⟨hal-02872101⟩

Share

Metrics

Record views

82

Files downloads

16